This is an educational reference tool — not medical advice. Nothing here constitutes a diagnosis, treatment, or prescription.
All dosing information reflects ranges cited in research and clinical literature. Always consult a licensed physician before any clinical application.
By entering, you confirm you are accessing this content for professional, educational, or research purposes and accept full responsibility for how you use it.
Not a consumer health app. For professional and research use only.
The PepRef
Stop Googling. Start Knowing.
Educational & Professional Reference Only
📱 Install The PepRef on your phone
Add it to your home screen for the full app experience — works offline, no app store needed.
1
Open ThePepRef.com in Safari or Chrome on your iPhone
2
Tap the Share button — the box with an arrow pointing up at the bottom of the screen (Safari) or the three dots menu (Chrome)
3
Tap "Add to Home Screen"
4
Tap "Add" — The PepRef icon now lives on your home screen.
1
Open in Chrome — works best in Chrome on Android
2
Tap the three dots menu in the top right corner of Chrome
3
Tap "Add to Home Screen" or "Install app" if you see that option
4
Tap "Add" — The PepRef icon now lives on your home screen.
Most peptides work with your cells. Bioregulators work on your DNA.
Regular peptides signal through surface receptors. Bioregulators penetrate the cell nucleus and restore youthful gene expression — organ by organ.
Chelohart restores the heart. Endoluten the pineal gland. Testoluten the testes.
Optimize vs restore. That's the difference.
40 years of clinical research. Hundreds of millions of administrations. No serious adverse events on record. Recognized by the FDA as dietary supplements.
Most Western medicine is only now catching up.
Educational reference only. Not medical advice. For professional and research use only.
Curated compound combinations by goal. Tap any stack to expand.
Educational reference only. Not medical advice. Consult a licensed physician.
Getting Started
Everything you need before your first vial.
Educational reference only. Not medical advice. Consult a licensed physician before any clinical application.
What is a peptide?
Peptides are short chains of amino acids — the same building blocks that make up proteins. Your body produces hundreds of them naturally as signaling molecules. As we age, many decline. Peptide research explores whether supplementing specific peptides can restore these signals for healing, performance, longevity, and cognitive function.
Most research peptides come as a white powder in a sealed vial. Before use they need to be reconstituted — mixed with bacteriostatic water to create a solution. This guide walks you through everything.
What you need
💧
Bacteriostatic Water (BAC Water) — 30mL
The only water you should use to reconstitute peptides. The 0.9% benzyl alcohol content prevents bacterial growth and keeps your peptide stable for 28–30 days refrigerated. Never use tap, distilled, or saline.
Standard for subcutaneous peptide injections. U-100 matches the reconstitution calculator. 29 gauge is thin enough that most people find it nearly painless. New syringe every injection — no exceptions.
Sterilize your vial tops and injection site before every use. Wipe and let dry 10–15 seconds — injecting through wet alcohol stings. Keep a pack with your peptides at all times.
💡 Storage: Refrigerate at 2–8°C before and after reconstitution. Keep away from light. Do not freeze. Stable for 28–30 days once reconstituted.
Reconstitution — step by step
Clean space. Washed hands. Follow these every time.
1Gather your supplies
You need: your peptide vial, BAC water vial, a fresh insulin syringe, and alcohol prep wipes. Lay them on a clean surface. Wash your hands thoroughly with soap and water before touching anything.
✓ Check your peptide vial — the powder should be white or off-white. If it looks discolored or smells unusual, do not use it.
2Calculate how much BAC water to add
Go to the Reconstitution tab in this app. Enter your peptide amount in mg, the amount of BAC water you plan to add, and your desired dose in mcg. The calculator will tell you exactly how many units to draw up per dose. A common starting point is adding 2 mL of BAC water to a 5 mg vial — this gives you a concentration of 2,500 mcg per mL.
✓ Write down your concentration on a small label and stick it to the vial. You'll thank yourself later.
3Sterilize both vial tops
Take an alcohol wipe and firmly wipe the rubber stopper on top of your BAC water vial. Take a second alcohol wipe and do the same to your peptide vial. Let both dry for 10–15 seconds. This step prevents contamination — never skip it.
⚠ Use a fresh wipe for each vial. Don't reuse wipes or touch the rubber tops with your fingers after wiping.
4Draw up the BAC water
Open a fresh insulin syringe. Push the needle through the rubber stopper of the BAC water vial. Turn the vial upside down so the liquid is at the bottom. Pull the plunger back slowly to draw up the amount of BAC water you calculated in Step 2. Check for air bubbles — if you see any, gently tap the syringe and push them out.
✓ Draw up slightly more than you need, push any air out, then pull back to your exact amount.
5Add the BAC water to the peptide vial
Insert the needle through the rubber stopper of your peptide vial. Angle the needle so it points toward the inside wall of the glass — not directly at the powder. Push the plunger slowly and let the water run down the side of the vial. This gentle approach prevents the peptide from degrading due to force or foam.
✗ Never squirt the water directly onto the powder with force. Never shake the vial. Both can damage the peptide structure.
6Dissolve the peptide
After adding the water, the powder may not dissolve immediately. Gently roll the vial between your palms or swirl it in slow circles until the solution is clear. This usually takes 30–60 seconds. Some peptides take longer — be patient.
✓ The finished solution should be clear or very slightly colored. If it looks cloudy or has particles floating in it after several minutes of gentle mixing, do not use it.
7Store correctly
Place your reconstituted vial in the refrigerator immediately. It will remain stable for 28–30 days at 2–8°C. Label it with the date you reconstituted it so you know when it expires. Keep it away from light — store it in the box it came in or wrap it in foil if needed.
⚠ Do not freeze reconstituted peptides. Freezing can damage the peptide structure. Freeze-drying (lyophilization) is done industrially under controlled conditions — a home freezer is not the same thing.
Injection basics
Most peptides are subcutaneous (subQ) — into the fat layer just under the skin. Some are intramuscular (IM) — into muscle. Check the compound entry for the correct route.
Subcutaneous (subQ) — most common
Inject into the fatty tissue just under the skin. Common sites are the abdomen (at least 2 inches from the belly button), the outer thigh, or the back of the upper arm. Pinch a small fold of skin, insert the needle at a 45-degree angle, and inject slowly. Most peptides in this library are administered subQ.
Intramuscular (IM) — some compounds
Injected directly into muscle tissue. Common sites are the outer thigh (vastus lateralis) or the deltoid (upper arm). Insert at a 90-degree angle. NAD+ is commonly referenced for IM administration. IM injection generally results in faster absorption than subQ.
Site rotation — always
Never inject in the same spot twice in a row. Rotating sites prevents scar tissue buildup (lipohypertrophy) which can affect absorption over time. If you inject in your abdomen, divide it mentally into quadrants and rotate through them.
→Drawing up your dose
Wipe the reconstituted peptide vial top with a fresh alcohol wipe. Let dry. Insert a fresh syringe needle through the stopper. Invert the vial. Draw back the plunger to the unit marking shown in the reconstitution calculator. Remove the needle, wipe your injection site with an alcohol wipe, let dry 10–15 seconds, then inject.
✓ Use the Reconstitution tab to calculate your exact unit marking every time. Never estimate.
This guide is for educational reference only. It does not constitute medical instruction. Consult a licensed healthcare provider before any clinical application. Improper injection technique carries real risk of infection and injury.
Reconstitution Calculator
Select a compound to auto-fill dosing, or enter manually.
Quick select compound
Peptide (MG)mg
BAC water to addmL
Reference dosemcg
Syringe type
Draw up
—
Volume
—
Doses / vial
—
Doses per bottle summary
Use sterile bacteriostatic water. Inject slowly down the vial wall — do not shake, gently swirl. Store at 2–8°C. Use within 28–30 days of reconstitution. New sterile syringe every use. Rotate injection sites.
For research and clinical preparation reference only.
mg ↔ mcg Converter
Type in either field to convert instantly.
Milligrams (mg)
⇄
Micrograms (mcg)
mg → mcg Quick Reference
1 mg = 1,000 mcg. Common peptide conversion reference.
Disclaimer
Educational and professional reference only. Not medical advice, diagnosis, or treatment.
Dosing ranges reflect values cited in peer-reviewed research and clinical literature. Clinical judgment required for any application.
Legal status varies by jurisdiction. Users bear full responsibility for compliance.
The creators assume no liability for any use or misuse of information presented.
Always consult a licensed physician before any clinical application.
On Bioregulators
Developed by the Khavinson Institute over 40+ years. Hundreds of millions of administrations. Recognized by the FDA as dietary supplements. Most Western research is only now catching up — but the safety record speaks for itself.
General considerations
Injection site reactions: Sterile technique is non-negotiable.
Hormonal effects: GH secretagogues may alter GH, IGF-1, cortisol, and insulin. Monitor accordingly.
Long-term data: Most compounds lack human safety data beyond 6–12 months.
Batch quality: Research-grade purity varies widely by supplier.
Oncologic: Growth-promoting compounds may interact with existing tumor biology.
GLP-1 class: Adequate calories, protein, and resistance training are non-negotiable to prevent lean mass and bone loss.
Risk by compound class — low to high
Educational reference only. Not medical advice. Consult a licensed physician before any clinical application.